Effect Of Ethanol Extract Of Desmodium Velutinum Stem On Some Marker Emzyme In Liver Of Albino Wistar Rats

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Abstract

Present investigations were carried out on the effect of ethanol extract of Desmodium velutinum stem on some liver enzymes of albino wistar rats fed with high fat from cow‟s brain. Twelve healthy albino wistar rats were divided into four groups. Group I rats were fed with balanced diet (normal feed, that is grower‟s mash) and tap water. Group II rats were fed with fat diet (cow‟s brain) (3.6g/day) alone. Group III rats received combined mixture of 70% fat diet (cow‟s brain) with 30% ethanol extract of Desmoduim elutinum stem (2.7g/day), Group IV rats were fed with fat diet (cow‟s brain) and were administered with a drug atorvastatin (5mg/rat/day).

The rats were sacrificed at the end of the experimental (two weeks) period. The plant extract was screened for its phytochemical constituents, using standard procedures. Phytochemical screening revealed the presence of tannins, flavonoids, saponins and alkaloids. The liver function tests was assessed by measuring the total bilirubin, total serum protein, direct and indirect bilirubin and liver enzymes such as Alanine amino transferase (ALT), Aspartate amino transferase (AST), Alkaline phosphate (ALP), Gamma-glutamyl transferase (GGT) on the liver of the albino wistar rat. The serum, ALT, ALP, AST and bilirubin concentration of the albino wistar rat increased significantly (P>0.05) compared to their total protein concentration.

The result obtained indicate that the ethanol extract of Desmodium Velutinum stem possess hepatoprotective activity on the fatty liver of albino wistar rat fed with fat from cow‟s brain. The studied plant showed variable amount of phytochemicals including flavonoid, alkaloid, saponin, tannins, terpenoid which were determined quantitatively using literature methods. The significant effect of the Desmodium velutinum stem ethanol extract was compared with standard drug, atorvastatin. The result in the present study suggest that the ethanol extract of Desmodium velutinum stem can be used in treating liver diseases.

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Keywords:
Ethanol extract, Desmodium velutinum, albino Wistar rats, Desmodium Velutinum Stem, Emzyme
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The conclusion section of the Effect Of Ethanol Extract Of Desmodium Velutinum Stem On Some Marker Emzyme In Liver Of Albino Wistar Rats summarizes the key findings, examines their significance, and may make recommendations or identify areas for future research

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