Antimicrobial Properties Of Chlorine And Alcohol Disinfectants

This study was carried out on the antimicrobial properties of chlorine and alcohol disinfectants. Disinfectant solutions were pre-pared according to manufacturers" instructions and tested against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Streptococcus mutans NCTC 1044, Candida albicans ATCC 90028, Bacillus subtilis ATCC 15244 spores, Mycobacterium tuberculosis ATCC 25177, My-cobacteriumavium subsp. avium ATCC 25291 and Hepatitis B virus using the Standard quantitative suspension test. The shelf-lives of the disinfectants were also determined. The results showed that both disinfectants killed all the test organisms within 30 seconds. B. subtilis spores were killed in 2 and 2.5 minutes by chlorine dioxide and sodium dichloroisocya-nurate respectively. When diluted solutions of these disinfectants were stored in screw cap bottles, they retained their activity for at least 30 days. The study concluded that Chlorine dioxide and sodium dichloroiso-cyanurate containing disinfectants can be used in the dental settings for surfaces and heat sensitive instruments. However, chlorine dioxide is advantageous because it is non-corrosive and the effective concentration is lower than that recommended for sodium dichloroisocyanurate.

The main objective of the study is to assess the antimicrobial properties of chlorine and alcohol disinfectants.

This study compares the antimicrobial effect of a chlorine dioxide and a chlorine generating disinfectant on the contaminants commonly present on dental instruments and in the dental surgery.

ABSTRACT

CHAPTER ONE
INTRODUCTION
1.1 Background of the study
1.2 Statement of the problem
1.3 Objectives of the study
1.4 Significance/ Justifications of the study

CHAPTER TWO
LITERATURE REVIEW
2.1 Concept of Chlorine
2.2 Properties of Chlorine
2.3 Chlorine as Sanitation, disinfection and antisepsis agent
2.3.1 Combating putrefaction
2.3.2 Disinfection
2.3.3 Semmelweis and experiments with antisepsis
2.3.4 Public sanitation
2.4 Concept of disinfectants
2.5 Alcohol as a disinfectant
2.6 Mechanisms of action

CHAPTER THREE
RESEARCH METHODOLOGY
3.1 Disinfectants
3.2 Test organisms and inocula
3.3 Test procedures for percentage kill
3.4 Hepatitis b virus
3.5 Shelf-life test

CHAPTER FOUR
RESULTS AND DISCUSSION
4.1 Results
4.2 Discussion

CHAPTER FIVE
CONCLUSIONS
REFERENCES

• Antimicrobial Properties Of Coconut Against Selected Bacterial And Fungal Species
• Studies On The Antimicrobial Properties Of Bark And Leaves Of Monkey Kola (Cola Millenii) On Some Selected Pathogens
• Antimicrobial Properties Of Bridialmacrantha Extracted By Various Solvents
• Synthesis And Antimicrobial Properties Of Palladium Chloride Complexes Of Rutin

• Microbiology Project Topics and Materials