Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed

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You are reading project material titled: Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed

Abstract
Elemental analysis is the qualitative detection and quantitative determination of chemical elements(atoms,ions)in a sample(Fritz Pregl 1923). To detect an
element, one should fix an appearance of an analytical signal. The formation of precipitate or characteristic crystals, colour change, an isolation of gaseous products, an appearance of a definite lines in spectrum, luminescence, etc. To determine elements quantity, it is necessary to measure a value of an analytical signal; a precipitate mass, intensity of a current, solution absorption, spectrum line, luminescence or radioactivity, a reaction rate and so on.(www.intechopen.com).
This study was undertaken to analse the elements present in grounded Pterocarpus mildbreadii (Oha seed) using Atomic Absorption Spectrometer(AAS MODEL-AA320N).The seed was found to contain these essential macro minerals/elements sodium(Na),Potassium(K),calcium(Ca),magnesium(Mg),and the trace elements iron(Fe),copper(Cu),zinc(Zn), and selenium(Se)(Duffus,2002).
The study established that Pterocarpus mildbreadii(oha seed) does not contain manganese and has high content of potassium which is necessary for good health.
Table of Content
Title page
Approval Page
Dedication
Acknowledgement
Table of Contents
Abstract

CHAPTER ONE
1.0 INTRODUCTION, AIMS & OBJECTIVES

1.1 Introduction
1.2 Aim of study
1.3 Research objective

CHAPTER TWO
2.0 LITERATURE REVIEW

2.1 Elemental analysis
2.2 Trace elements as catalysts
2.3 Biological functions of metals,sources and deficiency
2.4 Pterocarpus mildbreadii:A case study for elemental analysis
2.4.1 Systematic position of Pterocarpus mildbreadii
2.4.2 General characteristics of Leguminosae
2.4.3 Geographical distribution of Pterocarpus mildbreadii
2.4.4 Properties of Pterocarpus mildbreadii
2.4.5 Botany of Pterocarpus mildbreadii
2.4.6 Ecology of Pterocarpus mildbreadii
2.4.7 Management and propagation of Pterocarpus mildbreadii
2.4.8 Prospects of Pterocarpus mildbreadii
2.4.9 Uses of Pterocarpus mildbreadii

CHAPTER THREE
3.0 MATERIALS AND METHODS

3.1 Materials
3.1.1 Equipment/apparatus
3.1.2 Chemicals/reagents used
3.2 Methodology
3.2.1 The study area
3.2.2 Collection and preparation of plant material
3.2.3 Extraction
3.2.4 Preparation of reagent for AAS
3.3 Elemental analysis 39
3.4 Atomic Absorption Spectrometry

CHAPTER FOUR
4.0 RESULTS

4.0 Elemental content of Pterocarpus mildbreadii

CHAPTER FIVE
5.0 DISCUSSION

5.1 Conclusion
References

Chapter One
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CHAPTER ONE

1.1 INTRODUCTION, AIMS AND OBJECTIVES
A seed or mature ovule is a miniature plant with a protective cover in a suspended state of development. Most seeds contain a built-in food supply called endosperm, orchid is an exception. The endosperm can be made up of proteins, carbohydrates and fats.
Seed can also be defined as a small embryonic plant enclosed in a covering called the seed coat, usually with some stored food. It is the product of the ripened ovule of gymnosperm and angiosperm plants which occurs after fertilization and some growth within the mother plant(Wikipedia).Seed protects a plant embryo so that it can grow into a new plant. Many seeds are edible, such as sunflower seeds, tomato seeds, corn and peas.
Seeds contain three distinct structures. The inside of a seed contains an embryo, which is a baby plant with a shoot and a tiny root. The two halves of a seed are stored food that provides the nourishment necessary for seeds to germinate, or begin growing. Surrounding the seed is a hard, tough seed coat, which protects the seed during dormancy(Anville 2007).
Most seeds contain a built-in food supply called endosperm. The endosperm can be made up of proteins, carbohydrates and fats. Seeds also contain anti-nutrients in
their seed coat. These anti-nutrients includes phytin, lectin, trypsin inhibitor activity, tannin and cyanide. In addition, they also contain minerals such as sodium, potassium,calcium,magnesium,phosphorus,zinc,manganese,iron,selenium and copper.(Balogun 2000)
Oilseeds are energy dense foods; for example, sesame seeds provide 600kcal or 2470kj/1000g.Although oilseeds contain protein(|14-32g/100g)and carbohydrate(ranging from less than 1g/100g to more than 34g/100g),most of the food energy they provide is as fat(which provides 9kcal or 37kj/g).Oilseeds vary widely in their fatty acid composition but tend to be rich in MUFA(e.g peanut)r PUFA(e.g sunflower seeds).Some seed oils contain significant amounts of EFA, ALNA, an n-3 fatty acid, and linoleic acid(LA),an n-6 fatty acid. from these two fatty acids, the body can make all the fatty acids it needs. From LA, arachidonic acid can be produced, and from ALNA the long chain n-3 eicosapentaenoic acid(EPA)and docosahexanoic acid(DHA)can be made.(BNF 1999).
Generally, whole oilseeds are a source of fibre, phosphorus, iron and magnesium; many oilseeds are a source of vitamin E(an antioxidant),niacin and folate. Whole oilseeds also contain phytoestrogens, a group of substances including lignans and isoflavones. Phytoestrogens have a structure similar to the oestrogen hormone oestradiol and can bind to oestrogen receptors.Phytoestrogens may provide a protective effect against coronary heart disease as they have been shown to have a
lowering effect on blood cholesterol.Additionally,some phytoestrogens may have antioxidant properties(Goldberg 2003).
In Britain, oilseeds are usually consumed, following processing,as oils and margarines. The fatty acid composition of oils produced from oilseeds varies widely. Vegetable oils do not contain the same levels of macronutrients, vitamins and minerals as whole oilseeds. In fact, apart from fat itself, vitamin E is the only nutrient present in appreciable amounts. Vegetable oils do, however, contain a range of phytochemicals, e.g they are the main source of natural plant sterols in the diet. Plant sterols have a structure similar to cholesterol and hence reduce cholesterol absorption, therefore reducing the circulating levels of total and low density lipoprotein(LDL)cholesterol. Plant sterols can be present as free or esterified forms and the proportions vary, e.g free sterols dominate in soybean,olive and sunflower oil,while in rapeseed and corn oil, free sterols account for only 30% of the plant sterols.Refining vegetable oils decreases the content of sterols(from 10-70% depending on the oil and processing conditions used),thus decreasing their potential to lower serum cholesterol(Goldberg 2003).

1.2 AIM OF STUDY
To determine the elements present in grinded oha seed(Pterocarpus mildbreadii)

1.3 RESEARCH OBJECTIVE
This study was primarily designed to use grinded oha seed(Pterocarpus mildbreadii) extract for elemental analysis.


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Chapter Two
Chapter two of this Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed” research work is available. Order full work to download. Chapter Two of “Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed Contains: Literature Review, Elemental Analysis, Trace Elements As Catalysts, Biological Functions Of Metals,Sources And Deficiency , Pterocarpus Mildbreadii:A Case Study For Elemental Analysis, Systematic Position Of Pterocarpus Mildbreadii, General Characteristics Of Leguminosae, Geographical Distribution Of Pterocarpus Mildbreadii, Properties Of Pterocarpus Mildbreadii, Botany Of Pterocarpus Mildbreadii, Ecology Of Pterocarpus Mildbreadii, Management And Propagation Of Pterocarpus Mildbreadii, Prospects Of Pterocarpus Mildbreadii And Uses Of Pterocarpus Mildbreadii.
Chapter Three
Chapter three of this Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed” academic work is available. Order full work to download. Chapter Three of “Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed" Contains: Materials And Methods, Materials, Equipment/Apparatus, Chemicals/Reagents Used, Methodology, The Study Area, Collection And Preparation Of Plant Material, Extraction, Preparation Of Reagent For Aas, Elemental Analysis And Atomic Absorption Spectrometry.
Chapter Four
Chapter four of this Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed project work is available. Order full work to download. Chapter Four of Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed Contains: Results And Elemental Content Of Pterocarpus Mildbreadii.
Chapter Five
Chapter five of this Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed material is available. Order full work to download. Chapter Five of Elemental Analysis On Pterocarpus Mildbreadii (Oha)Seed Contains: Discussion, Conclusion And References.