Microbial Examination Of Spoiled Avocado Fruit

Microbial examination of spoilt Avocado fruits was carried out to obtained the causal organism. A total of 60 avocado from Ogbete Main market and New market Enugu were examined by culturing on nutrient agar and sabourand dextrose agar (SM), for bacteria and fungi causing post – harvest spoilage on the fruits; six bacterial species and six fungal species were isolated. The bacteria include Erwinia SP (66.7%), Streptococcus sp, *25%), E. Coli (18.9%) and staphycoccus sp (33.3%), Pseudomonas sp (20%) and Chromobacterium sp (5%) has the lowest percentage occurrence, Erwinia sp, Pseudomonas sp and chromobacterium sp were found to be absent in the control. The fungi species, includes Aspergillus sp (50%), Pericillium sp (25%), Fusarium sp (16.6%), Rhisopus sp (13.3%), currularia sp (8.3%) and Rhoctorula sp (3.3%), were also kolated Avocado fruits from Ogbete main market were more contaminated than the avocado form the new market.

1.0 INTRODUCTION
1.1 BACKGROUND OF STUDY
The Avocado pear, Persea Americana is a tropical and sub tropical fruit tree, originated in central America or adjoining regions of North or South America. It has now spread to much of the near tropical world. The avocado is limited especially by its climatic requirements, with their race differences. It is also highly susceptible to drought injury. But excess soil moisture is equally fatal, encouraging the dread phytophthora root rot in most of the world, and where the fungus is unknown. Despite the high water activity of most fruits, the how PH leads to their spoilage being dominated by fungi, both yeast and moulds. Other common diseases of pears include the black spot or scab caused by as comycetes
Avocado fruits has being estimated that one fourth of all produced harvested is no consumed before spoilage (Salulche, 1974), spoilage of fresh fruits usually occurs during storage and transport and while wacting to be processed unlike many other foods fruits after picking and before processing are ‘a live’ of the products plus the normal ripering process complicate an independent discussion of the microbiological spoilage problems are really market diseases of these products:
Shape, varies from slightly obligate to highly elongate, an intermediate oval or pear shape is preferred. Yellow to purple or black.
The composition of the fruit influences the likely type of spoilage. thus bacteria soft rot is widespread for the most part among the fruits is limited to those that are not highly acid. Because most fruits are somewhat acid are fairly dry at the surface, and are deficient in B vitamins, mold are the most common causes of spoilage. The compositions too, must determine the particular kind of molds most likely to grow, thus avocado support a large, variety of fungal spoilage organisms and other kinds comparatively few.

1.2 STATEMENT OF PROBLEM
Micro-organisms are known to destroy fruits, thereby reducing the quantity for consumption and the profits desirable from fruits such as avocado. Many spot are noticed on pears which do change the taste when consumed.
Bacteria and fungi are harmful and useful living organisms. The spoilage of most fruits is always associated with bactera and fungi, many diseses of plants are associated with bacteria and fungi (Micro-organisms)
The adverse effect of bacteria in plants and fruits has resulted in shortage of avocado fruits for consumption. Revenue is lost by farmers and industries that uses avocado fruits as a raw materials for fruit juice due to the spoilage caused by micro-organisms.

1.3 AIM AND OBJECTIVES OF THE STUDY
Microbial examination of spoilt avocado fruits
To isolate and identify how micro-organism destroy avocado so as to guide producer and farmers on how to manage the fruits
To isolate and identify it those micro-organisms are responsible for the spoilage
To compare the extent of spoilage in the area of colelction (tree) with that of market

1.4 HYPOTHESIS
HO: Microbial organisms do not cause spoilage of avocado at either in the field or during exposure for sale.
Hg = Microorganisms (Fungi and Bacteria) are responsible for avocado spoilage.

1.5 SIGNIFICANCE OF THE STUDY
This study will be useful to farmers, fruit juice industries and consumers of avocado fruits. T he outcome of this research will guide users of avocado fruit on the best method of preserving to avoid spoilage, the research work also will add to the literature and information existing about avocado for student and companies that uses avocado as raw materials for the production of fruit juice

1.6 LIMITATION OF THE STUDY
There are many or different type of microorganism that causes spoilage in avocado fruit or fruits in general, focus is made only on fungi and bacterial because they are the major organisms associated with spoilage in avocado fruits.

2.0 INTRODUCTION:

This chapter provides the background and context of the research problems, reviews the existing literature on the Microbial Examination Of Spoiled Avocado Fruit, and acknowledges the contributions of scholars who have previously conducted similar research [REV788] …

Title page
Certification
Dedication
Acknowledgement
List of Tables
List of figures
Abstract

CHAPTER ONE
Introduction
1.1 Background of study
1.2 Statement of problem
1.3 Aim and Objectives of the study
1.4 Hypothesis
1.5 Significance of the study
1.6 Limitation of the study

CHAPTER TWO
Literature review
2.1 The origin of Avocado fruits
2.2 Uses of avocado fruits
2.3 Preservation of avocado fruits
2.4 Ways avocado fruits can be prone to contamination
2.5 Microbial spoilage of avocado fruit
2.6 Control of bacterial and other disease of avocado fruits and fruits products
2.7 Some biochemical test for bacterial isolates

CHAPTER THREE
Materials and methods
3.1 Sample collection
3.2 Sterilization of media an material used
3.3 Preparation of media
3.4 Bacteria and fungi isolate from avocado fruits
3.5 Bacterial isolation and identification
3.6 Fungal isolation and identification
3.7 Lactophenole blue staining technique for fungi isolates
3.8 Staining techniques for bacteria isolates grains stain
3.9 Biochemical test for identification of bacteria isolates

CHAPTER FOUR
Results and discussion
4.1 Bacterial Examination
4.2 Fungi, examination

CHAPTER FIVE
Conclusion and recommendation
5.1 Conclusions
5.2 Recommendation
Reference

Document Information

  • Pages: 54 Pages
  • Chapters: 1 – 5 Chapters
  • Words: 7,239 Words
  • Format: DOC/PDF
  • Time: Instant Download
  • Title: Microbial Examination Of Spoiled Avocado Fruit:

YOU MAY LOVE THESE (Recommended)

To start a project on Production Of Mixed Fruit Using Fuse Locally Soured Citrus Fruits Orange Citrus Silences Tangerine Citrus Reticulate Lemon C Groups Citrus Paradox, follow these guidelines: Fix locally sourced citrus fruit (orange citrus sinners) tangerine (citrus reticulate) lemon citrus lemoni) (Lime (citrus aurantifolis) and Grape (citrus paradist) were mixed together to produce mixed fruit drink. Different ratios of orange, tangerine lemon, lime and Grape fruit juice in this ord…

61 Pages 1 - 5 Chapters 7,307 Words DOC/PDF Format Instant Download UPN2095

Understand how to write the “Microbial evaluation of ram milk from a Diary farm” Project

Posted under:

To develop a project on Microbial evaluation of ram milk from a Diary farm, follow these approaches: Microbial evaluation of twenty samples of raw milk from a diary farm (Emene fulani cattle rearers) was carried out using five method: viz direct microcopies count nutrient agar count, Blood agar count, Mac conkey agar count (celiforms only) and Acid fast bacilli staring was done to assay for the …

38 Pages 1 - 5 Chapters 5,193 Words DOC/PDF Format Instant Download UPN789

Discover how to write the “Microbial Contamination Of Yoghurt” Project

To undertake a project on Microbial Contamination Of Yoghurt, follow these effective ways: An in-depth assessment of the microbial contamination of selected yoghurt sold in Onitsha Market was conducted in this study using standard microbiological procedures. The study also evaluated the physical parameters of the yoghurt brands at the time of purchase. The results demonstrated that the…

61 Pages 1 - 5 Chapters 8,734 Words DOC/PDF Format Instant Download UPN4073

To write a project on Investigate The Microbial Content Of Some Bottled Soft Drinks Sold, follow these structure: This work was done in order to investigate the microbial content of some bolted soft drivels sold in Enugu interlopes. Soft drink are consumed in large quantities by the different age groups, adult and children of different class of people all over the state. This is aided by the general availabi…

49 Pages 1 - 5 Chapters 6,792 Words DOC/PDF Format Instant Download UPN780

Learn the structure to write the “Comparative Analysis Of Microbial Load In Water” Project

To carry out a project on Comparative Analysis Of Microbial Load In Water, follow these effective methods: Samples of water from the different hostels in campus II and other source of water production namely Ajali-owa water, from bore hole 9th mile and spring water from GRA water board were water collected and analyzed using total plate count and multiple tube fermentation techniques coliform. The res…

49 Pages 1 - 6 Chapters 5,928 Words DOC/PDF Format Instant Download UPN773

Posted under:

To start a project on Isolation And Identification Of Microorganism In Spoilt Meat (Cow), follow these guidelines: The nutrient and anti-nutrient content of raw millet, fermented and germinated millet flour were followed during a germination of 48h and a fermentation of 72hrs. There was a significant decrease in the anti-nutritional factors with tannins from 0.357%, to 0.157%, phytates 0.144% to 0.074% and an…

Live Chat