Antimicrobial Properties Of Chlorine And Alcohol Disinfectants

52 Pages 1 – 5 Chapters 6,850 Words

The Antimicrobial Properties Of Chlorine And Alcohol Disinfectants Project Material

Abstract

This study was carried out on the antimicrobial properties of chlorine and alcohol disinfectants. Disinfectant solutions were pre-pared according to manufacturers’ instructions and tested against Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 27853, Streptococcus mutans NCTC 1044, Candida albicans ATCC 90028, Bacillus subtilis ATCC 15244 spores, Mycobacterium tuberculosis ATCC 25177, My-cobacteriumavium subsp. avium ATCC 25291 and Hepatitis B virus using the Standard quantitative suspension test. The shelf-lives of the disinfectants were also determined. The results showed that both disinfectants killed all the test organisms within 30 seconds. B. subtilis spores were killed in 2 and 2.5 minutes by chlorine dioxide and sodium dichloroisocya-nurate respectively. When diluted solutions of these disinfectants were stored in screw cap bottles, they retained their activity for at least 30 days. The study concluded that Chlorine dioxide and sodium dichloroiso-cyanurate containing disinfectants can be used in the dental settings for surfaces and heat sensitive instruments. However, chlorine dioxide is advantageous because it is non-corrosive and the effective concentration is lower than that recommended for sodium dichloroisocyanurate.

Airms and Objectives

The main objective of the study is to assess the antimicrobial properties of chlorine and alcohol disinfectants.

This study compares the antimicrobial effect of a chlorine dioxide and a chlorine generating disinfectant on the contaminants commonly present on dental instruments and in the dental surgery.

Chapter One

1.0 INTRODUCTION
This chapter introduces the Antimicrobial Properties Of Chlorine And Alcohol Disinfectants and its relevance, states the research problems, research questions, and objectives, provides a background of the study, and should also include the research hypothesis.

Chapter Two: Literature Review

In this chapter, Antimicrobial Properties Of Chlorine And Alcohol Disinfectants is critically examined through a review of relevant literature that helps explain the research problem and acknowledges the contribution of scholars who had previously contributed immensely to similar research. The chapter intends to deepen the understanding of the study and close the perceived gaps …

Table of Contents

ABSTRACT

CHAPTER ONE
INTRODUCTION
1.1 Background of the study
1.2 Statement of the problem
1.3 Objectives of the study
1.4 Significance/ Justifications of the study

CHAPTER TWO
LITERATURE REVIEW
2.1 Concept of Chlorine
2.2 Properties of Chlorine
2.3 Chlorine as Sanitation, disinfection and antisepsis agent
2.3.1 Combating putrefaction
2.3.2 Disinfection
2.3.3 Semmelweis and experiments with antisepsis
2.3.4 Public sanitation
2.4 Concept of disinfectants
2.5 Alcohol as a disinfectant
2.6 Mechanisms of action

CHAPTER THREE
RESEARCH METHODOLOGY
3.1 Disinfectants
3.2 Test organisms and inocula
3.3 Test procedures for percentage kill
3.4 Hepatitis b virus
3.5 Shelf-life test

CHAPTER FOUR
RESULTS AND DISCUSSION
4.1 Results
4.2 Discussion

CHAPTER FIVE
CONCLUSIONS
REFERENCES

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