Isolation And Characterization Of Antibiotic Producing Actinomycetes In A Rhizosphere Environment

Abstract

This research work was carried out to isolation and characterization of antibiotic producing actinomycetes in rhizosphere environments using the standard microbiological method, crowded plate method, streak plate method and pour plate method. Starch casein agar and Nutrient agar were used for the characterization of the growth organisms and tests such as gram staining, starch hydrolysis, casein hydrolysis, lipid hydrolysis, citrate, methyl red, catalase and indole were carried out. After culturing only 5 out of the 13 isolates (from a total of 25 soil samples) showed visible growth and had antimicrobial activity on selected organisms(Stapylococus aureus, Bacillus subtilis and Escherichia coli), the total count for the colony forming units ranged from 3.9×106– 5.2×106.

The five isolates gotten from this work had four of them from the genus Streptomycetes (denoted as B, F, H and M) and the other from the genus Nocardia (L). Isolate B was active against Escherichia coli with a zone of inhibition measuring 26mm, isolate F, H, L and M were active against Staphylococcus aureus with zones of inhibition measuring 8mm, 9mm16mm and 9mm respectively, while isolate B, F, H, L and M were active against Bacillus subtitis with zones of inhibition measuring 5mm, 11mm, 11mm, 19mm and 7mm respectively. This study shows that Streptomyces are the most prevalent antibiotic producing actinomycetes in the soil. Therefore antibiotics should be taken only when needed to avoid antibiotic resistance by certain organisms and stored at appropriate conditions (temperature and pressure) hence, further purification, elucidation, and characterization are recommended to know the quality and novelty and commercial values of antibiotics.

Chapter One

1.0 INTRODUCTION:

This chapter introduces the Isolation And Characterization Of Antibiotic Producing Actinomycetes In A Rhizosphere Environment and its relevance, states the research problems, research questions, and objectives, provides a background of the study, and should also include the research hypothesis [INTRO50735]…

Chapter Two: Literature Review

2.0 INTRODUCTION:

This chapter provides the background and context of the research problems, reviews the existing literature on the Isolation And Characterization Of Antibiotic Producing Actinomycetes In A Rhizosphere Environment, and acknowledges the contributions of scholars who have previously conducted similar research [REV50735] …

Document Information

    • Format: DOC/PDF
    • Type: Project Material
    • Title: Isolation And Characterization Of Antibiotic Producing Actinomycetes In A Rhizosphere Environment:

YOU MAY LOVE THESE (Recommended)

  • Determination Of Prion Protein (PrP) And Changes In Fertility Hormones With Sleep Deprivation In Albino Rats

    To write about the Determination Of Prion Protein (PrP) And Changes In Fertility Hormones With Sleep Deprivation In Albino Rats research project, follow this helpful guideline: Wistar albino rats, numbering thirty five (35), were nurtured in the animal house of University of Nigeria Enugu Campus and used for this work. This work is designed to determine the presence of prion (PrP) in Wistar albino rats and the possible changes that sleep deprivation can cause in PrP and…

  • Construction Of An Open Steam Distillation Column

    To write about the Construction Of An Open Steam Distillation Column research project, follow this helpful guideline: We constructed an open steam distillation column with the following specifications:- FOR THE COLUMN Height of column = 500mm Length of pot = 50mm Diameter of pot = 5mm FOR THE STILL Port of thickness = 500mm Length of pot = 300mm Diameter of pot = 100mm FOR THE CONDESER Condenser thickness = 2mm …

    37 Pages 1 - 6 Chapters 4,030 Words DOC/PDF Format Instant Download UPN23759

  • Analysis Of Bore-Hole Water Quality Of Communities

    To write about the Analysis Of Bore-Hole Water Quality Of Communities research project, follow this helpful guideline: Water is one of the most important and abundant compounds of the ecosystem. All living organisms on the earth need water for their survival and growth. It rarely occurs in its pure form in nature (Ababio, 2005). It is the only substance that exists naturally on Earth in all three physical states …

  • Effect Of Telferia Occidentalis And Vernonia Amygdalina On Lipid Profile (Total Cholesterol, Triglyceride, HDL And LDL) On Brain, Plasma And Kidney In High Sucrose Diet Fed Rats

    To write about the Effect Of Telferia Occidentalis And Vernonia Amygdalina On Lipid Profile (Total Cholesterol, Triglyceride, HDL And LDL) On Brain, Plasma And Kidney In High Sucrose Diet Fed Rats research project, follow this helpful guideline: Vegetables and fruit provide a significant part of human nutrition, as they are important sources of nutrients, dietary fibre, and phytochemicals. Sixty (60) albino rats were used to study the effect vegetables extracts modified diets; grouped into, one group was fed with high sucrose diet while ot…

  • Isolation And Characterization Of Bioactive Compounds From Stembark Extract Of Uapaca Pilosahutch

    To write about the Isolation And Characterization Of Bioactive Compounds From Stembark Extract Of Uapaca Pilosahutch research project, follow this helpful guideline: Uapaca pilosa(Hutch.) a plant used in some parts of Africa in the treatment of dysentery, menstrual pain, fever, constipation, erectile dysfunction, skin infections, female sterility, pile, rheumatism, emetic, tooth-troubles and fatigue. The dried plant was extracted, the extract was subjected to…

Live Chat