Download Complete Antifungal Activity Of Locally Made Soap On Dermatophytes Isolated From Barbing Equipment Research Materials (PDF/DOC)
The comparative study on the effect of the locally made black soap and Dudu osun soap on dermatophytes isolated from barbing equipment using agar well diffusion method. The fungi isolated from the barbing equipment are Trichophyton rubrum (56.25%), Microsporum canis (28.13%), and Trichophyton mentagrophytes (15.63%). The isolates were treated with Dudu Osun and Black soap, the result showed that Trichophyton rubrum was resistance to 100% concentration of dudu soap but has a high growth level for 200, 300, and 400 concentration. In its treatment with black soap it was sensitive to all concentration. Trichophyton mentagrophyteswas sensitive to 100% and 400% concentration but has a high growth at 200% and 300% concentration in the treatment of dudu soap.
It showed sensitivity at 200 and 300 concentration and a high growth at 100 and 400 concentration for the treatment of black soap treatment. Microsporum canis was sensitive to dudu soap treatment at 100% and had a high growth at 200%, 300%, and 400% concentration. In its treatment with black soap. It showed sensitive results at 200% and 300% but a highly growth on 100% and 400%. All controls had maximum growth. The results obtained revealed that the traditional black soap exhibited a high level of antimicrobial activities resulting in greater zones of inhibition against dudu osun soap. Hence, African black soap through this study can thus, be recommended for use since it has potentials of treating hair and skin diseases.
Discussion, Conclusion And Recommendation
Discussion
The human hair harbors fungi, these fungi differs in their level of susceptibility to antimicrobial agents. The effect of soaps on the hair has not been widely studied. There is no soap that contains the required ingredient that suits all individual hair. Therefore reactions exhibited by individual hair differs from soap to soap. The different concentration tested on the different fungi isolated from human hair shows that the soaps contained antimicrobial activities which inhibited the growth of the fungi, T. rubrum, T. mentagrophytes and M. canisto different concentrations; 100%, 200%, 300% and 400%. The inhibition of the growth pattern of the isolates indicates the varying abilities of the fungi to resist the antimicrobial effect of the soaps. However these variations could be due to the differences in the nature and structures of the bacteria cell wall since it is the ultimate target of any antimicrobial agent or disinfectant. The result shows that the black soaps exhibited high levels of antimicrobial activity which is the ability of the soaps to inhibit the growth or destroy the organisms. The active ingredient in the soap is what distinguishes one type of the soap from another. The medicated soaps in this study were found to contain trichlocarban and triclosanas the active antimicrobial agents. These chemical compounds function by denaturing all disrupting cell activity and interfering with microbial metabolism. These depend on a number of factors such as the inherent properties of the organisms, contact time, the compostion of the soaps (e.gtriclosan), concentration of individual formulation and skin sensitivity. Traditional black soap lacks a key ingredient used in killing microorganisms such as triclosan, instead when the soap is scrubbed on the hair; it helps release oils on the surface of the hair that can kill and fungi bacteria and rinsing microorganisms away on the hair. It was also observed that black soap has the largest antimicrobial action against the isolates. However, the other soap sample (Dudu) possess very efficient antimicrobial agent but to a lesser degree.
Conclusion
In conclusion, The antifungi activity exhibited by Dudu and Black soap against dermatophytes isolated from barbing have provided scientific justification of their anti fungal activity.
Recommendation
I recommend that more attention should be given to the production of soaps or agents with antimicrobial effect as to help reduce the growth of microorganisms. Also, consumers should always use soap with antimicrobial effect to reduce the microbial load on human flora of the skin and hair.
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