Bacteriological Analysis Of Water Tanks
Abstract
Good quality drinking water can be polluted in many several ways of which faecal and soil pollution are the most important with respect to microbial agents. The aim of the study was to assess the contamination status of drinking water stored in water tanks in residence of Varitas University. Questionnaires were administered to residents to obtain information about the poly tanks and the usage of water from the tanks by users. Samples of the water tank were collected aseptically and 100 ml filtered through sterile membrane filters with 47 mm diameter and 0.45 µm pore size obtained from Merck Millipore. The membrane filters were aseptically placed on prepared sterile agar plates including, Harlequin agar for total coliforms and Escherichia coli, media-faecal coliforms (m-FC) for faecal coliforms, and plate count agar for total viable counts, respectively. The inoculated Harlequin and plate count agar plates were incubated at 37° for 24 h whiles the inoculated m-FC plate was also incubated at 44° for 24 h. From the questionnaires administered, 26% of the study participants said they use the water in the tank for drinking, 70% uses it for cooking, whiles all use it for bathing, and washing purposes. Mean counts of the indicator bacteria in the household storage water tanks ranged 2.5-3.5 log10 CFU/100 ml for total viable count, 2.3-3.2 log10 CFU/100 ml for total coliforms, 2.1-2.6 log10 CFU/100 ml for E. coli, and 0.5-2.9 log10 CFU/100 ml for faecal coliforms. From the study all the selected household storage water tanks were contaminated with faecal indicator bacteria including faecal coliforms and E. coli above the recommended World Health Organization (WHO) standards of concentration of faecal coliforms must be zero in safe drinking water. However, further studies are also recommended to be done to understand the effect of type of water storage tank (i.e.plastic, concrete, etc) on the water contamination.
Aims and Objectives
- To attain the total bacterial count of the water samples.
- To determine the coliform counts (Most Probable Number) of the water samples.
- To determine the species of bacteria present in the water.
Research Questions
- What are the types of microorganisms identified in water tank?
- What is microorganism?
- What type of bacteria is commonly found in water tank?
- What are the sickness that microorganism in the water can cause?
Chapter One
1.0 INTRODUCTION:
This chapter introduces the Bacteriological Analysis Of Water Tanks and its relevance, states the research problems, research questions, and objectives, provides a background of the study, and should also include the research hypothesis [INTRO66698]…
Chapter Two: Literature Review
LITERATURE REVIEW
2.1 OVERVIEW OF THE STUDY
Water is essential to life, but many people do not have access to clean and safe drinking water and many die of waterborne bacterial infections. In this review a general characterization of the most important bacterial diseases transmitted through water—cholera, typhoid fever and bacillary dysentery—is presented, focusing on the biology and ecology of the causal agents and on the diseases’ characteristics and their life cycles in the environment. The importance of pathogenic Escherichia coli strains and emerging pathogens in drinking water-transmitted diseases is also briefly discussed. Microbiological water analysis is mainly based on the concept of fecal indicator bacteria….
Table of Content
Cover page
Title page
Approval page
Dedication
Acknoweldgement
Abstract
Chapter one
1.0 Introduction
1.1 Background of the study
1.2 Problem statement
1.3 Aim of the study
1.4 Objective of the study
1.5 Significance of the study
1.6 Scope of the study
1.7 Purpose of the study
1.8 Limitation of the study
1.9 Research question
1.10 Definition of terms
Chapter two
Literature review
2.1 Overview of the study
2.2 Bacteriological water analysis
2.3 Review of bacteriological water analysis methodologies
2.4 Bacteria in water
2.5 Popular bacteria/virus treatment technology
2.6 Protecting water tanks from bacterial growth
Chapter three
3.0 Research methodology
3.1 Introduction
3.2 Ethical approval
3.3 Study area
3.4 Sample collection
3.5 Bacteriological analysis
3.6 Statistical analysis
Chapter four
4.1 Result and discussion
Chapter five
5.1 Conclusion
5.2 References